Table 2.
Astrocytes and neurons in WT and HOM Q175 mice and effects Ba2+ 200 μm
| Line | WT | HD | p | Test | % Difference | Meaning n | |||
|---|---|---|---|---|---|---|---|---|---|
| Mean ± SE | n | Mean ± SE | n | ||||||
| Astrocytes | |||||||||
| Basic properties | |||||||||
| 1 | Membrane potential without drugs (mV) | -80.7 ± 0.2 | 129 | -77.5 ± 0.5 | 103 | <0.0001 | MWT | -4 | Cells |
| 2 | Membrane potential with GluR blockers (mV) | -79.4 ± 0.5 | 140 | -75.8 ± 0.6 | 122 | <0.0001 | MWT | -5 | Cells |
| 3 | Membrane potential with GluR blockers + Ba 200 μm (mV) | -62.3 ± 1.5 | 30 | -57.5 ± 1.4 | 46 | <0.05 | tt | -8 | Cells |
| 4 | Membrane resistance (Rm = RIn - Rs) without drugs (MΩ) | 6.1 ± 0.4 | 55 | 7.2 ± 0.6 | 37 | NS | MWT | Cells | |
| 5 | Membrane resistance with GluR blockers + Ba 200 μm (MΩ) | 9.1 ± 1.2 | 14 | 10.0 ± 2.4 | 17 | NS | MWT | Cells | |
| 6 | Ba-induced change of membrane resistance (% CTRL) | 97.4 ± 13.3 | 14 | 43.6 ± 9.2 | 17 | <0.01 | tt | -55 | Cells |
| [Na+]i transients | |||||||||
| MP free (SBFI-AM, Rubi-Glu 3 mm) | |||||||||
| 7 | Asp-induced Na transient (% baseline) | 1.92 ± 0.12 | 73 | 0.84 ± 0.05 | 34 | <0.0001 | MWT | -56 | Cells |
| 8 | Rubi-Glu-induced Na transient (% baseline) | 1.39 ± 0.06 | 21 | 0.82 ± 0.07 | 28 | <0.0001 | tt | -41 | Cells |
| 9 | Ba-induced change of Rubi-Glu-induced Na transient (% CTRL) | -14.9 ± 4.7 | 21 | 1.3 ± 9.4 | 28 | NS | tt | Cells | |
| MP controlled (SBFI ic, Rubi-Glu 3 mm, focal stimulation of soma, Vh = -160 mV) | |||||||||
| 10 | Rubi-Glu-induced Na transient (% baseline) | 4.42 ± 0.32 | 16 | 3.32 ± 0.27 | 11 | <0.05 | tt | -25 | Cells |
| 11 | Ba-induced change of Rubi-Glu-induced Na transient (% CTRL) | -35.3 ± 5.7 | 10 | -30.7 ± 9.8 | 10 | NS | tt | Cells | |
| Kir4.1-related VAC and GTCs at [Rubi-Glu] 3 mm and Vh of -100 | |||||||||
| 12 | Amplitude of Ba-sensitive VAC at 100 mV (pA) | 1595 ± 189 | 21 | 793 ± 92 | 26 | <0.001 | MWT | -50 | Cells |
| 13 | Ba-induced GTC increment at Vh of -100 mV (pA) | 675 ± 45 | 21 | 404 ± 38 | 26 | <0.0001 | tt | -40 | Cells |
| 14 | Calculated GTC maximum in Ba Vh of -100 mV (pA) | 1959 ± 165 | 38 | 1432 ± 111 | 39 | <0.05 | F | -27 | Cells (pooled) |
| 15 | GTC centroid in Ba at Vh of -100 mV (ms) | 299 ± 12 | 11 | 341 ± 12 | 25 | <0.05 | tt | 12 | Cells |
| Neurons | |||||||||
| Synaptically induced GTC (cortical stimulation 1 mA) | |||||||||
| 16 | Mean GTC amplitude (pA) | 220 ± 42 | 18 | 217 ± 60 | 16 | NS | MWT | Cells | |
| 17 | GTC/EPSC ratio | 0.13 ± 0.02 | 10 | 0.04 ± 0.01 | 10 | <0.05 | tt | -69 | Cells |
| Corticostriatal EPSCs | |||||||||
| 18 | Half decay time (ms) | 3.58 ± 0.44 | 17 | 5.12 ± 0.33 | 14 | <0.05 | tt | +43 | Cells |
| Glu-induced spine currents (GISCs, Rubi-Glu 1 mm) | |||||||||
| 19 | Ba-induced change of GISC decay time constant (% CTRL) | 52.5 ± 5.9 | 9 | Cells | |||||
| Action potential-dependent sPSC frequency | |||||||||
| 20 | Frequency TTX-sensitive sIPSCs (s) | 0.39 ± 0.22 | 6 | 1.95 ± 0.41 | 3 | <0.05 | MWT | Cells | |
-
MWT, Mann-Whitney U test; tt, t test.